Single cell/single nucleus 3′ Gene Expression– The standard “workhorse” kit for single cell/nucleus RNA sequencing. If this happens, we will keep in touch with you to let you know when we receive the kits, so that you are not bringing over samples on a day when we do not have the reagents to process them. While 10X Genomics is usually able to deliver kits a day or two after they are ordered, they do suffer from occasional back-order issues. We ask that you provide a funding string at the time we place the order so that we can eventually bill for the cost of the kits even if your project plans do not end up proceeding.
For any other assay, we will order the kits specifically for your project. IMPORTANT: Deliverable data will be deleted from our systems 60 days after it is made available to download.Ī note about these kits: We only maintain a stock of the 3′ Gene Expression kits. Nuclei suspension can also be prepared and sequenced.70,000 reads per cell is appropriate for expression analysis. 50,000 reads per cell should be sequenced for saturating gene detection.Captures approximately 60% of loaded cells.10x Chromium instrument capable of capturing 500 to 10,000 cells per sample up to 80,000 cells per chip.Samples are barcoded individually for a multiplexed sequencing reaction.
8-channel microfluidic chip allowing up to 8 samples to be processed simultaneously.Please e-mail schedule a meeting to discuss your proposed experiment and timeline!
#10X CHROMIUM GEL BEAD SEQUENCE SOFTWARE#
The resulting data can then by analyzed and explored using the freely downloadable Loupe Cell Browser software ( link for Loupe Cell Browser free download, tutorial, and sample data sets).Īdditionally, 10x Genomics offers assays for single cell immune profiling (sc-V(D)J enrichment kit), single cell copy number variation profiling, single cell chromatin accessibility analysis (sc-ATAC-Seq), de novo assembly, and genome or exome sequencing. User provided single cell or single nuclei suspensions are loaded onto the Chromium iX to generate single cell barcoded gel-bead emulsions from which Illumina-compatible library preps are generated and sequenced on the NovaSeq in collaboration with UWBC DNA Sequencing Facility followed by optional analysis using a custom developed single cell data analysis pipeline by the UWBC Bioinformatics Resource Center. The Single Cell Gene Expression Assay is able to generate single cell transcriptomic measurements from up to 10,000 cells per cell suspension with up to 65% capture rate efficiency enabling the identification of rare cell types from heterogeneous populations. The 10x Genomics Chromium platform combines microfluidics with molecular barcoding to provide mRNA quantification from thousands of individual single cells.
0 kommentar(er)
